Service models
Experimental autoimmune encephalomyelitis (EAE)
C57BL/6 mice are widely used in the EAE model as they show a chronic and more gradual progress in disease compared to PL/J or B10.PL that show more acute and self-restrictive progress. Using this model, we provide efficacy evaluation services focused on clinical usefulness of candidate drugs targeting multiple sclerosis.
| Model | Evaluation interval | Evaluation items |
|---|---|---|
| C57BL/6 mice Wistar rats |
Daily |
|
Service analysis
Animal model
- EAE clinical score evaluation
- Weight changes
- Serum IgG, antigen-specific IgG, AQP4 autoantibody*
Brain and spinal cord tissues
- White matter inflammation and demyelination
- Axon damage (SMI312 IHC)
- T2 & T1-enhanced lesion*
- IHC test for CD3/19/68 in lesion*
- Detection of IgG/C9neo in lesion*
- GFAP stain for astrocyte damage*
Cytologic analysis of spleen and lymphatic gland
- Proliferation and death of target cells
- Analysis of 5 types of cytokine/chemokine* (open to negotiation with researcher)
- Cytometry (Th1/2/17, Treg)
- Analysis of 5 types of genetic markers (open to negotiation with researcher)
Immune cells derived from multiple sclerosis patients*
- Open to negotiation with researcher
